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为探究沙棘油莎豆复合饮料(STB)对盐酸洛哌丁胺诱导小鼠便秘的缓解作用，通过建立盐酸洛哌丁胺诱导小鼠便秘模型，将其随机分为阴性对照组[灌胃灭菌水0.2 mL/(d·只)]、模型组[灌胃10 mg/kg盐酸洛哌丁胺0.2 mL/(d·只)]、低剂量STB组[灌胃10 mg/kg盐酸洛哌丁胺0.2 mL/(d·只)+20.0 mL/kg STB 0.2 mL/(d·只)]和高剂量STB组[灌胃10 mg/kg盐酸洛哌丁胺0.2 mL/(d·只)+40.0 mL/kg STB 0.2 mL/(d·只)]，并测定小鼠体重、肠道推进率、血清胃肠神经递质水平、肠道组织形态及肠道菌群结构，评估STB的干预效果。研究结果显示，与对照组相比，模型组小鼠出现了显著(P<0.05)的便秘症状，说明建模成功。与模型组相比，低剂量STB和高剂量STB组粪便含水量显著提高至65%和60%(P<0.05)、肠道推进率提升至78%和75%(P<0.05)、首粒黑便排出时间缩短50%和38%(P<0.05)，并调节胃肠神经递质平衡。组织病理学结果显示，与模型组相比，STB可改善结肠隐窝结构，减少炎症浸润。16S rRNA测序分析表明，相比模型组，STB干预后肠道菌群中厚壁菌门丰度下降，拟杆菌门及有益菌属丰度显著增加(P<0.05)，菌群结构趋于健康状态。综上，STB通过促进肠道蠕动、调节神经递质及恢复菌群平衡缓解便秘，为功能性食品开发提供了理论依据。
Abstract：In order to investigate the relieving effect of a compound drink containing sea buckthorn and tiger nut composite beverage(STB) on loperamide hydrochloride-induced constipation in mice. A constipation model was established by administering loperamide hydrochloride,and the mice were randomly divided into 4 groups:the negative control group(gavage sterilized water 0. 2 mL/d per mouse),the model group(gavage 10 mg/kg loperamide hydrochloride 0. 2 mL/d per mouse),the low-dose STB group(gavage 10 mg/kg loperamide hydrochloride 0. 2 mL/d+20. 0 mL/kg STB 0. 2 mL/d per mouse),and the high-dose STB group(gavage 10 mg/kg loperamide hydrochloride 0. 2 mL/d+40. 0 mL/kg STB 0. 2 mL/d per mouse). The intervention effect of STB was evaluated by measuring body weight,intestinal propulsion rate,serum gastrointestinal neurotransmitter levels,intestinal tissue morphology,and intestinal flora structure. The results showed that compared with the control group,the model group exhibited significant(P<0. 05) constipation symptoms,indicating successful modeling.Compared with the model group,the low-dose STB and high-dose STB groups showed a significant increase in fecal water content(65% and 60%,P<0. 05),an increase in intestinal propulsion rate(78% and 75%,P<0. 05),a reduction in the time to first black stool excretion(50% and 38%,P<0. 05),and effective regulation of gastrointestinal neurotransmitter balance. Histopathological analysis revealed that STB improved the structure of colonic crypts and reduced inflammatory infiltration compared with the model group. 16S rRNA sequencing analysis showed that after the STB intervention,the abundance of Firmicutes in the intestinal flora decreased,while the abundance of Bacteroidetes and beneficial bacterial genera increased significantly,and the flora structure tended to be healthier compared with the model group. In conclusion,STB alleviated constipation by promoting intestinal motility,regulating neurotransmitters,and restoring flora balance,providing a theoretical basis for the development of functional foods.
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基本信息:
DOI：10.20088/j.cnki.jxau.2025.04.006
中图分类号:TS275.4
引用信息:
[1]雷雨彤,黄诗画,曾宏基,等.沙棘油莎豆复合饮料对盐酸洛哌丁胺诱导小鼠便秘的缓解作用[J].新疆农业大学学报,2025,48(04):304-312.DOI:10.20088/j.cnki.jxau.2025.04.006.
基金信息:
2023年新疆维吾尔自治区天池英才计划-青年博士(L)(2223RSTTCYC)
2025-11-14
2025
2025-11-26
2026-01-30
2026
1
2025-08-15
2025-08-15