| 82 | 0 | 166 |
| 下载次数 | 被引频次 | 阅读次数 |
根际作为植物?土壤?微生物发生复杂互作的核心界面，自其概念被提出以来，始终是土壤学领域的研究热点。植物通过根系持续向根际输入大量光合同化产物，进而调控土壤生物活性、关键生态过程及养分循环。当前，学界对根系分泌物的组成特征、产生机制及其在植物?微生物互作中的调控作用已形成较为清晰的认知。然而，针对根系分泌物如何影响土壤温室气体排放及其内在调控机制，目前尚未明确。本文系统梳理了根系分泌物影响温室气体排放和土壤微生物群落的研究工作，分别从根系分泌物的含量、种类及其类似物3个维度，探讨了根系分泌物介导的植物?微生物互作过程，及其对微生物驱动的温室气体排放的调控效应。在此基础上，进一步剖析了根系分泌物影响土壤微生物群落结构与温室气体排放的内在机制，明确指出当前研究存在的局限性，并对未来研究方向进行了展望。未来应进一步强化系统性与精细化的研究策略，尤其在全球气候变化背景下，需深入揭示根系分泌物对土壤碳、氮相关温室气体排放的贡献系数，并加强其对土壤碳库稳定性影响机制的研究，以期为精准调控土壤温室气体排放、提升土壤碳汇功能提供理论支撑。
Abstract：As the core interface of plant?soil?microorganism interaction,the rhizosphere has remained a research hotspot in soil science since its proposed. Plants release substantial photoassimilate into the rhizosphere via root systems,governing soil biological activity,ecological processes,and nutrient cycling. While a significant understanding exists regarding the composition and production mechanisms of root exudates,as well as the role in plant?microbe interactions,there remains a significant research gap concerning the influence on soil greenhouse gas emissions and the underlying mechanisms. This review systematically aims to summarize recent domestic research progress in understanding how root exudates influence soil microorganisms and greenhouse gas emissions.This paper systematically reviewed recent research progress on the influence of root exudates on greenhouse gas emissions and soil microbial communities and then further examined the root exudate?mediated plant?microbe interactions and their regulation of microbial?driven greenhouse gas production through three dimensions: the quantity of root exudates,the types of root exudates,and their analogs. Based on this foundation,the internal mechanisms by which root exudates affected soil microbial community structure and greenhouse gas emissions were further analyzed. The review also explicitly identified the limitations of current research and suggests potential directions for future studies. In the future,systematic and refined research strategies should be further strengthened,especially in the context of global climate change,the contribution coefficient of root exudates to soil carbon and nitrogen greenhouse gas emissions should be deeply revealed,and the research on its influence mechanism on the stability of soil carbon pool should be strengthened,in order to provide theoretical support for accurately regulating soil greenhouse gas emissions and improving soil carbon sink function.
[1]Panchal P,Preece C,Pe?uelas J,et al.Soil carbon sequestration by root exudates[J].Trends in Plant Science,2022,27(8):749-757.
[2]Friedlingstein P,O'Sullivan M,Jones M W,et al.Global carbon budget 2022[J].Earth System Science Data,2022,14(11):4811-4900.
[3]Jansson J K,Hofmockel K S.Soil microbiomes and climate change[J].Nature Reviews Microbiology,2020,18(1):35-46.
[4]Vives-Peris V,De Ollas C,Gómez-Cadenas A,et al.Root exudates:From plant to rhizosphere and beyond[J].Plant Cell Reports,2020,39(1):3-17.
[5]Yu Y,Zhou Y,Janssens I A,et al.Divergent rhizosphere and non-rhizosphere soil microbial structure and function in long-term warmed steppe due to altered root exudation[J].Global Change Biology,2024,30(1):17111.
[6]Pausch J,Kuzyakov Y.Carbon input by roots into the soil:Quantification of rhizodeposition from root to ecosystem scale[J].Global Change Biology,2018,24(1):1-12.
[7]Liu X J A,Sun J,Mau R L,et al.Labile carbon input determines the direction and magnitude of the priming effect[J].Applied Soil Ecology,2017,109:7-13.
[8]Faucon M P,Houben D,Lambers H.Plant functional traits:Soil and ecosystem services[J].Trends in Plant Science,2017,22(5):385-394.
[9]Jiang S,Ling N,Ma Z,et al.Short-term warming increases root-associated fungal community dissimilarities among host plant species on the Qinghai-Tibetan Plateau[J].Plant and Soil,2021,466(1):597-611.
[10]Hinsinger P,Plassard C,Tang C,et al.Origins of root-mediated pH changes in the rhizosphere and their responses to environmental constraints:A review[J].Plant and Soil,2003,248(1):43-59.
[11]Flessa H.Plant-induced changes in the redox potential of the rhizospheres of the submerged vascular macrophytes Myriophyllum verticillatum L.and Ranunculus circinatus L.[J].Aquatic Botany,1994,47(2):119-129.
[12]Xu X Y,McGrath S P,Meharg A A,et al.Growing rice aerobically markedly decreases arsenic accumulation[J].Environmental Science and Technology,2008,42(15):5574-5579.
[13]Zhang F,Shen J,Zhang J,et al.Rhizosphere processes and management for improving nutrient use efficiency and crop productivity:Implications for China[J].Advances in Agronomy,2010,107:1-32.
[14]Pascale A,Proietti S,Pantelides I S,et al.Modulation of the root microbiome by plant molecules:The basis for targeted disease suppression and plant growth promotion[J].Frontiers in Plant Science,2020,10:1741.
[15]Liljeroth E,Baath E,Mathiasson I,et al.Root exudation and rhizoplane bacterial abundance of barley(Hordeum vulgare L.)in relation to nitrogen fertilization and root growth[J].Plant and Soil,1990,127(1):81-89.
[16]Bais H P,Park S W,Weir T L,et al.How plants communicate using the underground information superhighway[J].Trends in Plant Science,2004,9(1):26-32.
[17]Hawes M C,Gunawardena U,Miyasaka S,et al.The role of root border cells in plant defense[J].Trends in Plant Science,2000,5(3):128-133.
[18]M?nchgesang S,Strehmel N,Trutschel D,et al.Plant-toplant variability in root metabolite profiles of 19 Arabidopsis thaliana accessions is substance-class-dependent[J].International Journal of Molecular Sciences,2016,17(9):1565.
[19]Chaparro J M,Badri D V,Vivanco J M.Rhizosphere microbiome assemblage is affected by plant development[J].The ISME Journal,2014,8(4):790-803.
[20]Gargallo-Garriga A,Preece C,Sardans J,et al.Root exudate metabolomes change under drought and show limited capacity for recovery[J].Scientific Reports,2018,8(1):12696.
[21]Ziegler J,Schmidt S,Chutia R,et al.Non-targeted profiling of semi-polar metabolites in Arabidopsis root exudates uncovers a role for coumarin secretion and lignification during the local response to phosphate limitation[J].Journal of Experimental Botany,2016,67(5):1421-1432.
[22]Qin R,Hirano Y,Brunner I.Exudation of organic acid anions from poplar roots after exposure to Al,Cu and Zn[J].Tree Physiology,2007,27(2):313-320.
[23]van Dam N M,Bouwmeester H J.Metabolomics in the rhizosphere:Tapping into belowground chemical communication[J].Trends in Plant Science,2016,21(3):256-265.
[24]Engedal T,Magid J,Hansen V,et al.Cover crop root morphology rather than quality controls the fate of root and rhizodeposition Cinto distinct soil Cpools[J].Global Change Biology,2023,29(19):5677-5690.
[25]Blagodatskaya E,Kuzyakov Y.Active microorganisms in soil:Critical review of estimation criteria and approaches[J].Soil Biology and Biochemistry,2013,67:192-211.
[26]Feng H,Fu R,Luo J,et al.Listening to plant's Esperanto via root exudates:Reprogramming the functional expression of plant growth-promoting rhizobacteria[J].The New Phytologist,2023,239(6):2307-2319.
[27]Martin F M,Uroz S,Barker D G.Ancestral alliances:Plant mutualistic symbioses with fungi and bacteria[J].Science,2017,356(6340):4501.
[28]Acharya S M,Yee M O,Diamond S,et al.Fine scale sampling reveals early differentiation of rhizosphere microbiome from bulk soil in young Brachypodium plant roots[J].IS ME Communications,2023,3(1):54.
[29]Ling N,Wang T,Kuzyakov Y.Rhizosphere bacteriome structure and functions[J].Nature Communications,2022,13(1):836.
[30]Berendsen R L,Pieterse C M,Bakker P A.The rhizosphere microbiome and plant health[J].Trends in Plant Science,2012,17(8):478-486.
[31]Zhalnina K,Louie K B,Hao Z,et al.Dynamic root exudate chemistry and microbial substrate preferences drive patterns in rhizosphere microbial community assembly[J].Nature Microbiology,2018,3(4):470-480.
[32]Gargallo-Garriga A,Preece C,Sardans J,et al.Root exudate metabolomes change under drought and show lim-ited capacity for recovery[J].Scientific Reports,2018,8(1):12 615-12696.
[33]Yuan J,Zhao J,Wen T,et al.Root exudates drive the soilborne legacy of aboveground pathogen infection[J].Microbiome,2018,6(1):156.
[34]Liu J,Li X,Jia Z,et al.Effect of benzoic acid on soil microbial communities associated with soilborne peanut diseases[J].Applied Soil Ecology,2017,110:34-42.
[35]Li X G,Jousset A,De Boer W,et al.Legacy of land use history determines reprogramming of plant physiology by soil microbiome[J].The ISME Journal,2019,13(3):738-751.
[36]Czaban W,Rasmussen J,Laursen B B,et al.Multiple effects of secondary metabolites on amino acid cycling in white clover rhizosphere[J].Soil Biology and Biochemistry,2018,123:54-63.
[37]Phillips R P,Finzi A C,Bernhardt E S.Enhanced root exudation induces microbial feedbacks to N cycling in a pine forest under long-term CO_2 fumigation[J].Ecology Letters,2011,14(2):187-194.
[38]薛志婧,屈婷婷,刘春晖,等.培养条件下枯落物分解过程中微生物残体对土壤有机碳形成的贡献[J].应用生态学报,2023,34(7):1845-1852.
[39]Cheng W,Parton W J,Gonzalez-Meler,et al.Synthesis and modeling perspectives of rhizosphere priming[J].New Phytologist,2014,201(1):31-44.
[40]Hopkins F,Gonzalez-Meler M A,Flower C E,et al.Ecosystem-level controls on root-rhizosphere respiration[J].New Phytologist,2013,199(2):339-351.
[41]Shi S,Richardson A E,O'Callaghan M,et al.Effects of selected root exudate components on soil bacterial communities[J].FEMS Microbiology Ecology,2011,77(3):600-610.
[42]Ferry J G.Methane from acetate[J].Journal of Bacteriology,1992,174(17):5489-5495.
[43]Sj?gersten S,Cheesman A W,Lopez O,et al.Biogeochemical processes along a nutrient gradient in a tropical ombrotrophic peatland[J].Biogeochemistry,2011,104(1):147-163.
[44]Girkin N T,Turner B L,Ostle N,et al.Root exudate analogues accelerate CO_2 and CH_4 production in tropical peat[J].Soil Biology and Biochemistry,2018,117:48-55.
[45]Wright E L,Black C R,Cheesman A W,et al.Impact of simulated changes in water table depth on ex situ decomposition of leaf litter from a neotropical peatland[J].Wetlands,2013,33(2):217-226.
[46]Hamer U,Marschner B.Priming effects in different soil types induced by fructose,alanine,oxalic acid and cat-echol additions[J].Soil Biology and Biochemistry,2005,37(3):445-454.
[47]Paterson E,Gebbing T,Abel C,et al.Rhizodeposition shapes rhizosphere microbial community structure in organic soil[J].New Phytologist,2007,173(3):600-610.
[48]Brooks Avery G,Shannon R D,White J R,et al.Controls on methane production in a tidal freshwater estuary and a peatland:Methane production via acetate fermentation and CO_2 reduction[J].Biogeochemistry,62(1):19-37.
[49]Lovley D R,Coates J D,Blunt-Harris E L,et al.Humic substances as electron acceptors for microbial respiration[J].Nature,1996,382(6590):445-448.
[50]Lipson D A,Jha M,Raab T K.Reduction of iron(III)and humic substances plays a major role in anaerobic respiration in an Arctic peat soil[J].Journal of Geophysical Research:BioGeosciences,2010,115(G4):G00106.
[51]Ye R Z,Jin Q S,Bohannan B,et al.pH controls over anaerobic carbon mineralization,the efficiency of methane production,and methanogenic pathways in peatlands across an ombrotrophic-minerotrophic gradient[J].Soil Biology and Biochemistry,2012,54:36-47.
[52]Lopez-Hernandez D,Siegert G,Rodriguez J V.Competitive adsorption of phosphate with malate and oxalate by tropical soils[J].Soil Science Society of America Journal,1986,50(6):1460-1462.
[53]Renella G,Egamberdiyeva D,Landi L,et al.Microbial activity and hydrolase activities during decomposition of root exudates released by an artificial root surface in Cdcontaminated soils[J].Soil Biology and Biochemistry,2006,38(4):702-708.
[54]Jones D L,Dennis P G,Owen A G,et al.Organic acid behavior in soils-misconceptions and knowledge gaps[J].Plant and Soil,2003,248(1):31-41.
[55]Ladha J K,Tirol-Padre A,Reddy C K,et al.Global nitrogen budgets in cereals:A 50-year assessment for maize,rice and wheat production systems[J].Scientific Reports,2016,6(1):19355.
[56]马芬,杨荣全,郭李萍.控制氮肥施用引起的活性氮气体排放:脲酶/硝化抑制剂研究进展与展望[J].农业环境科学学报,2020,39(4):908-922.
[57]Bardon C,Piola F,Bellvert F,et al.Evidence for biological denitrification inhibition(BDI)by plant secondary metabolites[J].New Phytologist,2014,204(3):620-630.
[58]Coskun D,Britto D T,Shi W,et al.How plant root exudates shape the nitrogen cycle[J].Trends in Plant Science,2017,22(8):661-673.
[59]Sun L,Lu Y,Yu F,et al.Biological nitrification inhibition by rice root exudates and its relationship with nitrogen-use efficiency[J].New Phytologist,2016,212(3):646-656.
[60]Weston L A,Ryan P R,Watt M.Mechanisms for cellular transport and release of allelochemicals from plant roots into the rhizosphere[J].Journal of Experimental Botany,2012,63(9):3445-3454.
[61]于海洋,杨玉婷,马静,等.硝化抑制剂对覆膜稻田CH4和N_2O排放的影响[J].生态环境学报,2017,26(3):461-467.
[62]李豫婷,林树基,韩雪,等.CO_2浓度升高与硝化抑制剂对冬小麦田间N_2O排放量的影响[J].生态学报,2016,36(15):4762-4768.
[63]Mangalassery S,Mooney S J,Sparkes D L,et al.Impacts of zero tillage on soil enzyme activities,microbial characteristics and organic matter functional chemistry in temperate soils[J].European Journal of Soil Biology,2015,68:9-17.
[64]Velmourougane K,Venugopalan M V,Bhattacharyya T,et al.Soil dehydrogenase activity in agro-ecological sub regions of black soil regions in India[J].Geoderma,2013,197:186-192.
[65]Jones D L,Murphy D V.Microbial response time to sugar and amino acid additions to soil[J].Soil Biology and Biochemistry,2007,39(8):2178-2182.
[66]Trevors J T.Effect of substrate concentration,inorganic nitrogen,O_2 concentration,temperature and pH on dehydrogenase activity in soil[J].Plant and Soil,1984,77(2):285-293.
[67]Srinivasan K,Mahadevan R.Characterization of proton production and consumption associated with microbial metabolism[J].BMC Biotechnology,2010,10:2.
[68]Wang Z P,Delaune R D,Patrick Jr,et al.Soil redox and pH effects on methane production in a flooded rice soil[J].Soil Science Society of America Journal,1993,57(2):382-385.
[69]Hunger S,Schmidt O,Hilgarth M,et al.Competing formate-and carbon dioxide-utilizing prokaryotes in an anoxic methane-emitting fen soil[J].Applied and Environmental Microbiology,2011,77(11):3773-3785.
[70]王伯豪,刘丹阳,马合巴丽·奥腊孜别克,等.外源有机酸对盐碱土壤苗期棉花根系生理特征及根系分泌物中有机酸的影响[J].新疆农业大学学报,2023,46(5):385-393.
[71]Sun H,Jiang S,Jiang C,et al.A review of root exudates and rhizosphere microbiome for crop production[J].Environmental Science and Pollution Research,2021,28(39):54497-54510.
[72]Sokol N W,Kuebbing S E,Karlsen-Ayala E,et al.Evidence for the primacy of living root inputs,not root or shoot litter,in forming soil organic carbon[J].New Phytologist,2019,221(1):233-246.
[73]Girkin N T,Turner B L,Ostle N,et al.Root exudate analogues accelerate CO_2 and CH_4 production in tropical peat[J].Soil Biology and Biochemistry,2018,117:48-55.
[74]Girkin N T,Turner B L,Ostle N,et al.Composition and concentration of root exudate analogues regulate greenhouse gas fluxes from tropical peat[J].Soil Biology and Biochemistry,2018,127:280-285.
[75]Zhou J,Wen Y,Shi L,et al.Strong priming of soil organic matter induced by frequent input of labile carbon[J].Soil Biology and Biochemistry,2021,152:108069.
[76]Villarino S H,Pinto P,Jackson R B,et al.Plant rhizodeposition:A key factor for soil organic matter formation in stable fractions[J].Science Advances,2021,7(16):3176.
[77]Rugova A,Puschenreiter M,Koellensperger G,et al.Elucidating rhizosphere processes by mass spectrometry:A review[J].Analytica Chimica Acta,2017,956:1-13.
[78]Sasse J,Martinoia E,Northen T.Feed your friends:Do plant exudates shape the root microbiome?[J].Trends in Plant Science,2018,23(1):25-41.
[79]Shen Z,Ruan Y,Xue C,et al.Soils naturally suppressive to banana Fusarium wilt disease harbor unique bacterial communities[J].Plant and Soil,2015,393(1):21-33.
[80]Lópezráez J A,Shirasu K,Foo E.Strigolactones in plant interactions with beneficial and detrimental organisms:The Yin and Yang[J].Trends in Plant Science,2017,22(6):527-537.
基本信息:
DOI：10.20088/j.cnki.jxau.2025.02.001
中图分类号:X144
引用信息:
[1]贾宏涛,李典鹏,买迪努尔·阿不来孜,等.根系分泌物对土壤温室气体排放影响的研究进展[J].新疆农业大学学报,2025,48(02):83-92.DOI:10.20088/j.cnki.jxau.2025.02.001.
基金信息:
国家自然科学基金项目(31560171)